Introduction: Formalin-fixed, paraffin embedded (FFPE) tissue specimens are highly valuable sources for retrospective studies of many pathologies. Nevertheless, the extraction of nucleic acids from FFPE specimens could often be challenging, as nucleic acids become cross-linked and degraded during the archiving process. Nucleic acids obtained are usually highly fragmented and chemically modified from the archiving process.
• No toxic chemicals
• No lost of nucleic acids due to filtering washes
• Short and robust protocol
• No inhibition on downstream applications
BioChains FFPE Tissue DNA Extraction Kit allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues, with potential high throughput capabilities and full compatibility for down-stream applications such as qPCR. Utilizing heat and proteinase K treatment, BioChains FFPE Tissue DNA Extraction Kit is optimized in the removal of paraffin, partial reversal of formalin crosslinking, and release of DNA from fixed tissues.
Content: All necessary reagents for DNA extractions in FFPE tissue specimens are provided. The kit contains sufficient reagents for 100 FFPE tissue DNA extraction reactions. Quality Control All kit components are DNase-, RNase-, and protease-free. Each component has been tested for purity and efficacy.
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