bioWORLD* bioSPECTRUM* Dye-affinity Column Set

The bioSPECTRUM™ Dye-affinity Test Set is a kit with six different columns containing agarose bead-immobilized dyes that makes it easy to evaluate whether one of the six has selective affinity for the protein(s) of interest. Dye affinity is a rapid, inexpensive, and versatile method that is applicable to the purification of many proteins. Dye chromatography uses mimics of natural protein ligands to act aspseudo-affinity ligands for protein separations. Reactive dyes are immobilized to a solid support, Separopore 4B-CL matrix. The dyes act as a competitive inhibitor for a proteins normal ligand.

Several reactive textile dyes have been found to bind to proteins, especially those with affinities to various nucleotides. Immobilized dyes have been found to bind from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate / cofactor similarities, as well as hydrophobic and ion exchange properties. Because the affinity or specificity of a dye for a particular nucleotide binding site is not very well predicted, the most effective method for the determination of a specific proteins binding capacity is a screening procedure with several types of immobilized reactive dyes.

bio-WORLDs bioSPECTRUM™ Dye-affinity Test Set includes a trial size, six-pack pre-packed column of 2.5 ml each:
Red-Separopore 4B-CL
Blue-Separopore 4B-CL
Reactive blue 4-Separopore 4B-CL
Yellow-Separopore 4B-CL
Green-Separopore 4B-CL
Brown-Separopore 4B-CL
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Size: 6 Column