Description
Pre-activated medium for immobilization of ligands containing primary amines. N, N-Carbonyl diimidazole (CDI) mediated activation of Separopore matrix to allow coupling of the primary amino ligands by a stable amide bond formation. No charge is introduced into the matrix after the coupling reaction. No ligand leakage after coupling. Superior stability: Activated matrix is much more resistant to hydrolysis. Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids. Coupling reaction can also be carried out in organic solvents. High coupling efficiency and easy to handle. CDI-activated Separopore 6B-CL is activated with CDI groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles.
Technical specifications:
• Group to be coupled: -NH2 groups
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 m
• Molecular weight range: 6 x 104 - 2 x 107
• Coupling conditions: pH: 8.5 - 11, Temp: 4 - 25 °C, Time: 6 - 24 h
• pH stability: 11 (ligand dependent)
• Storage: 2 - 8 °C
• Supplied as suspension in acetone
Image(s) are representative of the product group and not necessarily the individual product.
Size: 5 mL
Size: 25 mL
Size: 100 mL
Size: 250 mL
Size: 500 mL