bioWORLD* CNBr-activated Separopore* Agarose 4B

Cyanogen bromide-activated Separopore 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore 4B to form cyanate esters or imidocarbonates. These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore matrix.

Highlights:
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Multi-point attachment of many protein ligands, resulting in a chemically stable product.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.

Technical specifications:
• Group to be coupled: -NH2
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 m
• Molecular weight range: 6 x 104 - 2 x 107
• Binding capacity: 10 - 25 mg -chymotrypsinogen / ml drained gel
• pH Stability: 2 - 11 (ligand dependent)
• Coupling conditions: pH 9 - 10, Temp: 4 - 25 DEG C, Time: 4 - 16 h
• Storage: 2 - 8 °C
• Supplied as lyophilized powder (with additives for stabilization)
• 1g swell to 5 ml
Image(s) are representative of the product group and not necessarily the individual product.

Size: 1 g

Size: 5 g

Size: 10 g