bioWORLD* Glutathione Separopore* 4B-CL Super Flow (SF)

Reduced glutathione is covalently linked Separopore 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Glutathione Separopore Super Flow (SF) is used for protein purification by batch/column and scale-up procedures. Glutathione Separopore Super Flow (SF) has an excellent protein binding capacity. The good flow properties of the 92 m (average size) highly crosslinked Separopore matrix makes an excellent choice for protein purification by column purification or batch process for scale-up. Storage: 2-8°C.

This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated 4% crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST-fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.
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Size: 25 mL

Size: 100 mL

Size: 500 mL