bioWORLD* Arachis hypogaea (Peanut) Agglutinin-Separopore* 4B

Applications:
Arachis hypogaea (Peanut Agglutinin, PNA) Separopore 4B-CL is used for the purification of O-linked glycoproteins.

Peanut agglutinin is a 110,000 molecular weight lectin composed of four identical subunits of approximately 27,000 daltons each. PNA binds preferentially to a commonly occurring structure, galactosyl (-1,3) N-acetylgalactosamine. This carbohydrate sequence (called the T-antigen) is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding has been employed as a measure of cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte human subpopulations and animals, and to measure the levels of lymphoid cell populations in many diseases. Presence of Ca++ can enhance PNA binding.

Technical Specifications:
• Ligand source: Arachis hypogaea (peanut Lectin).
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52-165 µm
• Matrix activation: Cyanogen bromide
• Matrix attachment: Amino
• Ligand density: 5 mg Peanut lectin/ml drained gel.
• Binding Capacity: 0.5-1.5 mg of asialofetuin / ml of drained medium.
• Inhibiting/Eluting Sugar: 200 mM galactose.
• Carbohydrate for Elution: 0.1M lactose or 0.2M galactose in buffer. 10-50 mM N-acetylgalactosamine may also be used.
• Supplied as suspension in 1M NaCl containing 0.01% thimerosal.
• pH stability: 4-9
• Storage: 2-8 °C
• Application note: Calcium is required for binding. Ca2+ concentration should not exceed 0.5 mM in the buffer (to not form a white precipitate).

Products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
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Size: 10 mL

Size: 25 mL