acumedia* BCYE Agar Base

• Intended Use: BCYE Agar Base is used for the isolation of Legionella spp
• Product Summary: In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires disease, a
multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L.
pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and
replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar.4 In 1980, Pasculle et al. reported that
CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer.5 One year later,
Edelstein further increased the sensitivity of the medium by adding the potassium salt of alpha-ketoglutaric acid
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