bioWORLD* ECH Separopore* (Agarose) 4B

Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups. Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The matrix provides a 10-atom spacer arm convenient for coupling of small ligands. No blocking reaction is necessary following the coupling reaction. Ideal for immobilization of small ligands stable in a low pH and containing primary amines. Coupling reaction can be carried out in organic solvent.

Technical specifications:
• Matrix active group: -COOH groups
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 m
• Molecular weight range: 6 x 104 - 2 x 107
• Group to be coupled: -NH2 groups
• Chemical linkage of ligand: Stable amide linkage
• Ligand density : 12 - 16 mol carboxyl groups / ml drained matrix
• Spacer arm: 6-amino caproic acid, 10 atoms
• Coupling conditions pH 4.5 - 6, Temp: 4°C - 25 °C, Time: 1.5 - 24 h; organic solvents possible
• Storage: 2 - 8 °C
• Supplied as pre-swelled gel suspension in buffered saline
Image(s) are representative of the product group and not necessarily the individual product.

Size: 5 mL

Size: 15 mL

Size: 25 mL

Size: 50 mL