acumedia* Presence-Absence Broth

• Intended Use: Presence-Absence Broth is used for the detection of coliform bacteria in water treatment plants or distribution
systems using the presence-absence coliform test.
• Product Summary and Explanation: The Presence-Absence (P-A) test is a presumptive detection for coliforms in water. The test
is a simple modification of the multiple-tube procedure. One 100 ml test sample is inoculated into a single culture bottle to obtain
qualitative information on the presence or absence of coliforms, through the presence or absence of lactose fermentation. This
test is based on the principle that coliforms and other pollution indicator organisms should not be present in a 100 mL water
sample.

Comparative studies with the membrane filter procedure indicate the P-A test may maximize coliform detection in samples containing many organisms that could overgrow coliform colonies and cause problems in detection. The P-A test is described in standard methods for water testing and U.S. EPA.
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