bioWORLD* 2-Iminobiotin

2-Iminobiotin-Separopore 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore than the biotin resin. It is also used for removal of free avidin from (strept)avidin conjugate preparations. Interaction with avidin and streptavidin is pH dependent: forms stable complex above pH 9.5 and dissociates at pH 4.

Technical specifications:
• Ligand: 2-Iminobiotin
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Amino
• Molecular weight range: 6 x 104 - 2 x 107
• Binding capacity: 4 mg avidin / ml drained gel
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as suspension in 0.5M NaCl, 0.01M sodium phosphate (pH 6.8) and 0.02% sodium azide
• Appearance: White to off-white gel suspension
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Size: 5 mL

Size: 10 mL