bioWORLD* Calmodulin Separopore* (Agarose) 4B

Calmodulin-Separopore 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding site. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme substrate calmoldulin-Ca2+ complexes are particularly stable.

Technical specifications
• Ligand: Calmodulin (Bovine)
• Matrix: Separopore 4B (agarose beads, 4 %)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix activation: Cyanogen bromide
• Ligand density: 1 mg calmodulin / ml drained gel
• Matrix attachment: Amino
• Matrix spacer: 1 atom
• pH stability: 4 - 9
• Storage: 2 - 8 °C
• Chemical stability: Stable to all commonly used aqueous solutions
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
Image(s) are representative of the product group and not necessarily the individual product.

Size: 1 mL

Size: 5 mL

Size: 10 mL