bioWORLD* EAH Separopore* (Agarose) 4B

EAH-Separopore 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), bio-Worlds EAH-Separopore 4B is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Technical specifications:
• Matrix active groups: -NH2 groups
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 m
• Matrix activation: Epoxy
• Group to be coupled: -COOH groups
• Chemical linkage of ligand: Stable amide linkage
• Spacer arm: 6-amino caproic acid, 10 atoms
• Ligand density: 7 - 11 mol amino / ml medium
• Coupling condition: pH 4.5 - 6, Temp: 4 - 25 °C
• Storage: 20% ethanol containing 0.9% NaCl
• Storage temperature: 4 - 8 °C
Image(s) are representative of the product group and not necessarily the individual product.

Size: 5 mL

Size: 15 mL

Size: 25 mL