bioWORLD* Epoxy-activated Separopore* (Agarose) 6B

Used in coupling of hydroxyl, amino or thiol groups to Separopore 6B-CL via a 12-carbon hydrophilic spacer arm.
Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates. Epoxy-activated Separopore 4B-CL is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group. Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.

Technical specifications:
• Matrix active group: Epoxy
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 m
• Group to be coupled: -NH2, -OH, or -SH
• Molecular weight range: 6 x 104 - 2 x 107
• Chemical linkage of ligand: Ether
• Matrix spacer: 12 atoms (when ligands are coupled to free epoxy groups. Linkage is stable, uncharged ether)
• Coupling conditions: pH 9 - 10 (amino ligands), pH 11 - 12 (hydroxyl ligands), pH 7.5 - 8.5 (thiol ligands); Temp: 4 - 37 °C; Time: 16 h to several days; organic solvents possible
• pH stability: 2 - 11 (ligand dependent)
• Storage: 2 - 8 °C
• Supplied as lyophilized powder stabilized with lactose and dextran
• 1g swells to 3 - 4 ml
Image(s) are representative of the product group and not necessarily the individual product.

Size: 1 g

Size: 5 g

Size: 10 g