bioWORLD* Glutathione Separopore* (Agarose) 4B-Lyophilized

Reduced glutathione is covalently linked Separopore 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated 4 % crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST -fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.

Technical specifications:
• Ligand: Reduced glutathione (GSH)
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix activation: Epoxy
• Matrix spacer: 12 atoms
• Ligand density: 5 - 10 mg GST / ml drained gel
• Binding capacity: 5 - 10 mg recombinant GST-tagged protein / ml drained gel
• pH stability: 4 - 13
• Storage: 4 °C - 30 °C
• 1 Gram lyophilized powder swells 10 ml of gel
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Size: 1 g

Size: 5 g