bioWORLD* GTP-Separopore* (Agarose) 4B

GTP-Separopore is used for purification of GTP-dependent enzymes and proteins that bind nucleotides and related molecules. The GTP-Separopore 4B-CL resin comprises GTP attached to agarose beads via its -phosphate. A long hydrophilic spacer (14-atom) is used to minimize unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. Linkage of GTP via the terminal phosphate means that the immobilized ligand cannot be removed by phosphatases in crude extracts. Affinity resins such as GTP-Separopore 4B-CL are been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules.

Technical specifications:
• Ligand: Guanosine 5-triphosphate (GTP)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Ligand density: 8 - 12 mM GTP / ml drained gel
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as lyophilized powder stabilized with lascose. 1 g will swell approximately 5-7 ml.
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Size: 1 mL

Size: 5 mL

Size: 25 mL