bioWORLD* NHS-activated Separopore* (Agarose) 4B

Rapid immobilization of antibodies and other proteins to the matrix. Immobilization of ligands through primary amines to purify recombinant proteins. Immobilization of Protein A or Protein G for monoclonal antibody purification. Pre-activated medium for immobilization of ligands containing a primary amino group. N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands. The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling. Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes. Coupling reaction can also be carried out in organic solvents. Provides a 10 atom spacer arm upon ligand coupling. Coupling completed after 2 - 4 h at room temperature. NHS-Activated Separopore provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.

Technical specifications:
• Group to be coupled: Primary -NH2
• Matrix: Separopore 4B-CL (highly crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 m
• Molecular weight range: 6 x 104 - 2 x 107
• Chemical linkage of ligand: Amide bond
• Spacer arm: 6-aminocaproic acid, 10 atoms
• Substitution: >18 mol NHS / ml drained gel
• pH stability: 3 - 13 (ligand dependent)
• Coupling conditions: pH 6 - 9; Temp: 4 - 25 °C
• Storage: 2 - 8 °C
• Flow rate specifications: 70 - 140 cm / h
• Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups
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Size: 5 mL

Size: 10 mL

Size: 25 mL

Size: 50 mL