bioWORLD* Ni2+-Chelated Separopore* (Agarose) 4B

Ni2+-chelated Separopore 4B-CL is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to nickel metal ions. Ni2+-chelated Separopore 4B-CL is ideal for purification of recombinant proteins containing (His)6 fusion tag.

Technical Specifications:
• Chelating group: Nickel
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Matrix activation: Epoxy
• Particle size range: 52 - 165 µm
• Metal ion capacity: >20µmol Ni2+ / ml drained gel
• Binding capacity: 5 - 8 mg (His)6-tagged protein / ml medium
• pH stability: 2 - 11
• Chemical stability: For one week at 40 °C: 0.01M HCl, 0.1M NaOH
For 12 h: 1M NaOH, 70% acetic acid.
For 30 min tested: 30% 2-propanol.
For 1 h tested: 2% SDS
• Compatibility during use: Stable in all commonly used buffers, reducing agents, denaturants, and detergents
• Supplied as suspension in 20% ethanol
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Size: 10 mL

Size: 25 mL

Size: 50 mL