bioWORLD* Protein A-Separopore* (Agarose) 4B

Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. Protein A-Separopore 4B-CL is used for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. This is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.

Technical Specifications
• Ligand: Native protein A (from Staphylococcus aureus, 42 kDa)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Molecular weight range: 6 x 104 - 2 x 107
• Ligand density: 3 mg Protein A / ml drained gel
• Binding capacity: 20 mg human IgG / ml drained gel
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Storage: 2 - 8 °C
• Supplied as lyophilized powder
Image(s) are representative of the product group and not necessarily the individual product.

Size: 1 g

Size: 5 g