bioWORLD* Protein G-Separopore* (Agarose) 4B

Protein G binds specifically to Fc regions of many mammalian immunoglobulins. Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments. Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method. Protein G-Separopore 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.

Technical Specifications
• Ligand: Native Protein-G from Streptococcus sp.
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Matrix activation: Cyanogen bromide
• Particle size range: 52 - 165 µm
• Exclusion limit range: 6 x 104 - 2 x 107
• Ligand density 2 mg Protein-G / ml drained medium
• Binding capacity: 20 mg human IgG / ml drained gel
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Storage: 2 - 8 °C
• Supplied as suspension in 0.5M NaCl
Image(s) are representative of the product group and not necessarily the individual product.

Size: 1 mL

Size: 5 mL

Size: 10 mL