bioWORLD* Reactive Blue-4 Separopore* (Agarose) 4B

Reactive Blue 4-Separopore is a dye affinity matrix used to purify proteins based on selective binding interactions between certain protein sites and the dye molecule. Different immobilized dyes bind from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive dyes, such as Reactive Blue 4,to proteins may be due to substrate/cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins. Reactive Blue 4-Separopore 4B-CL separates proteins based on affinity for this blue dye.

Technical Specifications:
• Ligand: Reactive Blue 4 dye
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Triazine coupling
• Ligand density: 2 - 5 mg reactive blue / ml drained gel
• Binding capacity: 5 mg human serum abumin / ml drained gel
• pH stability: 3 - 13
• Storage: 2 - 8 °C
• Supplied as 50% slurry, 0.5M NaCl containing 0.02% thimerosal
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Size: 5 mL

Size: 25 mL