bioWORLD* Streptavidin-Separopore* (Agarose) 4B

Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications.
The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligand and the sample.
Strptavidin-Separopore 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.

Technical specifications
• Ligand: Native Streptavidin (from Streptomyces avidinii)
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 -165 µm
• Matrix activation: Cyanogen bromide
• Ligand density: 2 mg streptavidin / ml drained gel
• Binding capacity: >20 µg biotin / ml drained gel
• Exclusion limit: 2 x 107
• pH stability: 3 - 10
• Binding buffer: 50 mM ammonium carbonate, 0.5M NaCl, pH 10
• Elution buffer: 50 mM ammonium acetate, 0.5M NaCl, pH 4
• Supplied as suspension in buffered saline solution.
• Storage: 2 - 8 °C
Image(s) are representative of the product group and not necessarily the individual product.

Size: 1 mL

Size: 5 mL

Size: 10 mL