bioWORLD* Tosyl-activated Separopore* (Agarose) 4B

Tosyl-activated Separopore 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interfering with the antigen binding region.

Technical specifications:
• Matrix active group: p-toluenesulfonyl chloride (tosyl chloride)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 m
• Groups coupled to: Primary amines at pH range 9 - 10
• Phosphate, HEPES, bicarbonate and borate buffers should be used
• Binding capacity: 4 - 8 mg chymotrypsin /ml matrix
• Storage: 2 - 8 °C
Image(s) are representative of the product group and not necessarily the individual product.

Size: 10 mL

Size: 50 mL

Size: 100 mL