bioWORLD* Ubiquitin-Separopore* (Agarose) 4B

Ubiquitin-Separopore 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore 4B is used in affinity chromatography to study enzymes involved in intracellular protein degradation, calpain and lysosomal proteases, proteomics, and ubiquitination analysis. Ubiquitin- Separopore 4B has been used in a study to produce evidence for a particulate location of ubiquitin conjugates and ubiquitin-conjugating enzymes in the rabbit brain. Ubiquitin- Separopore 4B has also been used to study fertilization of the ascidian, Halocynthia roretzi. Ubiquitin is covalently linked to Separopore beads via primary amine coupling leaving the C-terminus free.

Technical Specifications:
• Ligand: Ubiquitin (bovine)
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size: 52-165 µm
• Matrix spacer: 6 carbon
• Ligand density: 7 - 15 mg ubiquitin / ml drained gel
• Binding Capacity: 2 mg protein /ml drained gel
• Storage: 4 °C
• Supplied as suspension in 1M NaCl containing 0.02% thimerosal
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Size: 2 mL

Size: 10 mL