bioWORLD* viewIP* His-Tag Nickel Red Separopore* (Agarose)

Affinity matrix that is red colored, immobilized metal-ion affinity chromatography (IMAC) designed for use in small scale affinity capture (molecular pull-down) experiments. Red IMAC gel consists of quadridentrate chelate bound with nickel and covalently attached to Red beaded 6% agarose (Separopore 6B). View-IP™ His-tag Nickel Red-Separopore 6B-CL is convenient for small-scale affinity capture of fusion proteins with histidine containing tags and greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.

Technical Specifications:
• Ligand: Nitrilotriacetic acid (NTA)
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size (range): 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Binding capacity: approximately 15 mg His-tagged protein/ml drained gel
• Exclusion limit: approximately 2 x 107
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Supplied as 50% suspension in PBS
• Storage: 2 - 8 °
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Size: 1 mL