Thermo Scientific* Acclaim* HILIC-10 LC Columns

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific Acclaim HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5-20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.

Rugged and Reproducible Separations of Highly Polar Compounds:
• Unique selectivity, complementary to reversed-phase columns
• Enhanced sensitivity for MS detection
• Hydrolytically stable
• Rugged column packing
• Broad application range, including separation of hydrophilic drugs and drug metabolites

Acclaim HILIC-10 LC columns are available in 3m particle size and a variety of column formats: 4.6 x 150 mm, for high-throughput routine analysis; 3 x 150 mm, for high-throughput analysis with reduced solvent consumption; and 2.1 x 150 mm, for LC-MS analysis.
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Flow Rate (mL/min.): 0.2 to 0.4
Max Pressure (psi): 8500
Particle Size (um): 3
Length (mm): 150
Diameter (mm): 2.1

Flow Rate (mL/min.): 0.4 to 0.8
Max Pressure (psi): 8500
Particle Size (um): 3
Length (mm): 150
Diameter (mm): 3

Flow Rate (mL/min.): 0.8 to 1.5
Max Pressure (psi): 8500
Particle Size (um): 3
Length (mm): 150
Diameter (mm): 4.6