Thermo Scientific* Acclaim* PepMap* 100 C18 LC Analytical Columns, NanoViper

Generate high-resolution analyses of tryptic, natural, and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns. These columns are often used for LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. Due to their high loading capacity, Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples. Most of these C18 columns are pre-assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation. Classic columns are available as well.

Column Fittings
• Analytical columns are supplied with a standard ¹/32 in. fitting at inlet
• nanoViper columns are supplied with a nanoViper fitting at inlet and has 10 mm usable fused silica on the outlet
• nanoViper FS columns are supplied with a nanoViper fitting at inlet and has 45 mm usable fused silica at outlet for greater compatibility with Peek sleaves and microtight unions

Key Advantages of nanoViper Fittings
• Ease of use, no sleeve for small capillaries
• Tool-free assembly
• No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third party valves and unions
• No experimental failure due to bad connections

Ideal for high-resolution peptide mapping of tryptic, natural and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery and systems biology.
• High loading capcity
• Use for the analysis of low abundant peptides often found in complex proteomics samples
• Select the column length and I.D. for your throughput, sensitivity and amount of sample available requirements
• Choose from 50, 150, 250, 500 mm lengths and 50, 75, 300 and 1000 µm I.D.
• Superior protein and peptide separations
• Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
• Outstanding separation efficiency
• Superior resolution and recovery
• Easy installation with nanoViper™ Fingertight Fittings

PepMap nano columns are also available pre-assembled with Thermo Scientific Dionex nanoViper Fingertight Fitting for a dead-volume-free connection to 1000 bar.
• Requires no tools
• Provides virtually zero-dead-volume connections
• Withstands pressures up to a 1000 bar
Image(s) are representative of the product group and not necessarily the individual product.

Flow Rate: 6 uL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 150
Diameter (mm): 0.3

Flow Rate: 6 uL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 50
Diameter (mm): 0.3

Flow Rate: 200 nL/min.
Max Pressure (bar): 500
Particle Size (um): 3
Length (mm): 150
Diameter (mm): 0.075

Flow Rate: 200 nL/min.
Max Pressure (bar): 500
Particle Size (um): 3
Length (mm): 150
Diameter (mm): 1

Flow Rate: 200 nL/min.
Max Pressure (bar): 500
Particle Size (um): 3
Length (mm): 250
Diameter (mm): 0.075

Flow Rate: 200 nL/min.
Max Pressure (bar): 500
Particle Size (um): 3
Length (mm): 500
Diameter (mm): 0.075

Flow Rate: 200 nL/min.
Max Pressure (bar): 500
Particle Size (um): 3
Length (mm): 50
Diameter (mm): 0.075

Flow Rate: 300 nL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 150
Diameter (mm): 0.05

Flow Rate: 300 nL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 150
Diameter (mm): 0.75

Flow Rate: 300 nL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 50
Diameter (mm): 0.05

Flow Rate: 300 nL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 50
Diameter (mm): 0.75

Flow Rate: 300 nL/min.
Max Pressure (bar): 800
Particle Size (um): 2
Length (mm): 50
Diameter (mm): 1