Thermo Scientific* ProSwift* RP-4H Capillary Monolithic HPLC Columns

For fast, high-resolution LC-MS separations in protein identification, biomarker discovery, and systems biology applications, use Thermo Scientific ProSwift Monolithic HPLC Columns. Based on a ethylvinylbenzene divinylbenzene copolymer, the monolithic structure offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages to the chromatographic separation. High-sensitivity proteomics and biotech applications are easily performed using these columns.

Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size.

Medium Channel (H) Size (permeability)
These channels and the monoliths non-porous surfaces provide fast mass transfer for the high-resolution and fast protein separations. The monolith morphology results in low operating back pressure, allowing the use of higher linear velocities which can improve resolution of large biomolecules.
• High-speed protein separations
• High sensitivity for LC/MS
• High column-to-column reproducibility
• Wide range of column I.D. and lengths available
• Superior lifetime
• Easy column installation using Thermo Scientific Dionex nanoViper Fingertight Fittings
• Precolumns Available to Preconcentrate and Desalt Samples

You can also use Thermo Scientific PepSwift Trap Columns for preconcentration and desalting of samples consisting of peptides and proteins without negatively impacting the chromatographic performance or recovery of the compounds. Change the selectivity of the separation or improve the trapping efficiency by employing various ion-pairing agents in the loading solvent and/or mobile phases. The trap columns are used with Thermo Scientific PepSwift and ProSwift Monolithic Columns, which are for fast, high-resolution LC/MS separations in protein identification, biomarker discovery, and systems biology applications.
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Flow Rate: 2 to 12 µL/min.
Max Pressure (psi): 4950
Particle Size: Monolith
Length (mm): 250
Diameter (mm): 0.2

Flow Rate: 12 to 80 µL/min.
Max Pressure (psi): 2000
Particle Size: Monolith
Length (mm): 100
Diameter (mm): 0.5

Flow Rate: 500 to 3000 nL/min.
Max Pressure (psi): 4950
Particle Size: Monolith
Length (mm): 250
Diameter (mm): 0.1

Flow Rate: 500 to 3000 nL/min.
Max Pressure (psi): 4950
Particle Size: Monolith
Length (mm): 500
Diameter (mm): 0.1