Analtech* VPF Apparatus with or without Hot Plate

The Analtech Vapor Phase Fluorescence Chamber is a new advance in the fields of qualitative and quantitative TLC. Difficult-to-detect compounds, such as sugars, can now be located at the nanogram level through chemically induced fluorescence. VPF is a general fluorogenic visualization reaction accomplished by heating the TLC plate in the presence of ammonium bicarbonate. Fluorescence is one of the most sensitive and easily quantitated TLC detection methods. The visualization chamber is invaluable for sulfuric acid charring and other detection techniques requiring heat for the formation of derivatives.

The chamber is very useful for activation and evaporation of residual mobile phase. The unit consists of an aluminum block heat distributor surrounded by a stainless steel frame and non-asbestos insulation. The device mounts on a standard 6.5 X 6.5 inch hot plate (included in Spectrum Cat. No. 476-11905) and insures rapid rise to the set temperature and even heat distribution. A thermometer extends from the aluminum core to allow accurate reading of temperature for reproducible analysis. The TLC plate rests directly on the heat distributor and is enclosed within a narrow vapor space to insure maximum density of reagent vapors.

A PYREX window allows the reaction to be followed for optimal color development. A tubular port permits the introduction or removal of vapors and doubles as a plate removal device.
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