acumedia* EC Medium, Modified

• Intended Use: EC Medium, Modified is used with novobiocin for the selective pre-enrichment of Escherichia coli O157:H7.
• Product Summary: EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the
coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture.
Growth of spore-forming bacteria and fecal streptococci were inhibited by the bile salts. EC Medium, Modified with the addition
of novobiocin was first described by Okrend and Rose. Okrend and Rose modified EC Medium by reducing the Bile Salts Mixture
concentration to 1.12% and adding 20 mg/L of sodium novobiocin. Okrend and Rose et al. reported this formulation, which they
called Modified EC & Novobiocin (mEC&N), was beneficial in the enrichment and detection of E. coli O157:H7 from meats and
poultry, and is currently recommended by the U.S.D.A.
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