acumedia* Nutrient Gelatin

• Intended Use: Nutrient Gelatin is used for the differentiation of microorganisms on the basis of gelatinase production.
• Product Summary and Explanation: Gelatin was the first gelling agent used to solidify culture media. The advantages of solid media over liquid media include isolation of pure cultures and the ability to perform plate counts. The disadvantages of gelatin include incubation at 20°C, a temperature that is lower than optimum for growing many microorganisms, and the fact that many organisms metabolize (liquefy) gelatin. Agar later replaced gelatin as a solidifying agent.
Identifying fermentative and non-fermentative Gram-negative bacilli include testing for gelatin liquefaction. If the proteolytic enzyme gelatinase is present, gelatin is hydrolyzed and loses its gelling characteristic.
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