acumedia* Phenol Red Broth Base

• Intended Use: Phenol Red Broth Base is used with carbohydrates for the differentiation of microorganisms on the basis of
carbohydrate fermentation reactions.
• Product Summary and Explanation: The fermentative properties of bacteria are valuable criteria in their identification. A basal
medium for determining the fermentation reactions of microorganisms must be capable of supporting the growth of test
organisms and free from fermentable carbohydrates. Vera used a fermentation test medium employing the pH indicator phenol red
and obtained accurate results.

Phenol Red Broth Base is recommended for use to determine the ability of organisms to ferment various carbohydrates. Various fermentable substances may be added in any desired concentration. The concentration of carbohydrate generally employed for testing fermentation reactions of bacteria is 0.5 to 1%. Some investigators prefer to use 1% rather than 0.5% to ensure against reversion of the reaction due to depletion of the carbohydrate.
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