acumedia* Rappaport-Vassiliadis R10 Broth

• Intended Use: Rappaport-Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods.
• Product Summary and Explanation: Rappaport et al1 formulated an enrichment medium for Salmonella spp. that was modified by
Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis
modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later
showed that incubation at 41.5 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a
selective enrichment medium that is used following pre-enrichment of the specimen. It has gained approval for use in analyzing
milk and milk products, raw flesh foods, highly contaminated foods, and animal feeds. This medium selectively enriches for
Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green,
high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.
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