acumedia* Rappaport-Vassiliadis Salmonella Enrichment Broth

• Intended Use: Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella
spp. Conforms to Harmonized USP/EP/JP Requirements.
• Product Summary and Explanation: Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by
Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis
modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later
showed that incubation at 41.5 0.5°C for 24 hours improved recovery of Salmonella spp.

Rappaport-Vassiliadis Salmonella Enrichment Broth is a modification of Rappaport-Vassiliadis R10 Broth, where Soy Peptone has replaced Enzymatic Digest of Casein as the nitrogen and vitamin source. Soy Peptone has shown to enhance the growth of Salmonella spp. and minimizes Bovine Spongiform Encephalopathy (BSE) risk. This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.
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