acumedia* Tergitol 7

• Intended Use: Tergitol 7 Agar is used for the selective isolation of coliform bacteria.
• Product Summary and Explanation: Tergitol 7 Agar, prepared according to the formula published by Chapman, is selective for
Escherichia coli and members of the coliform group. Chapman reported that the addition of Tergitol 7 to a medium consisting of
polypeptone and yeast extract permitted unrestricted development of all coliform bacteria, and inhibited Gram-negative spore-
formers and Gram-positive organisms. Coliform counts on Tergitol 7 Agar were 30% higher than on other selective media.

Chapman modified his original Tergitol 7 Agar by adding 40 mg of triphenyltetrazolium chloride (TTC) per liter. This medium was helpful in the early recognition and identification of Escherichia coli. Confirmation of the presence of E. coli was possible after only 10 hours incubation at 35°C. Chapman also reported that Tergitol 7 Agar with added TTC gave a selective medium suitable for the isolation of Candida spp. and other fungi. Tergitol 7 Agar with TTC is useful for routine water analysis and the examination of foods.
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