acumedia* Tryptic Soy Broth

• Intended Use: Tryptic Soy Broth is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to
Harmonized USP/EP/JP Requirements.
• Product Summary and Explanation: Tryptic Soy Broth, a general purpose medium, is commonly referred to as Soybean-Casein
Digest Medium, and abbreviated as TSB. This medium was originally developed for use without blood in determining the
effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow
luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in
cosmetics and complies with established standards in the food industry. TSB was chosen by the USDA Animal and Plant Health
Inspection Service for detecting viable bacteria in live vaccines.

Tryptic Soy Broth is recommended by the National Committee for Clinical Laboratory Standards (NCCLS) for inoculum preparation in disk diffusion sensitivity tests. The rich nutritional base of TSB, supplemented with SPS and CO2, is an excellent broth for blood cultures in clinical applications. With the addition of 6.5% NaCl, TSB can be used for the selective growth of group D streptococci.
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