acumedia* Violet Red Bile Agar

• Intended Use: Violet Red Bile Agar is used for the enumeration of coliforms in food and dairy product conforms to American
Public Health Association (APHA).
• Product Summary and Explanation: The coliform group of bacteria includes aerobic and facultative anaerobic, gram-negative,
non-sporeforming bacilli. Coliforms ferment lactose and form acid and gas at 35°C within 48 hours. Members of
Enterobacteriaceae comprise the majority of the group, but other lactose fermenting organisms may also be included.

Procedures to detect, enumerate, and presumptively identify coliforms are used in testing foods and dairy products. One method for performing the presumptive test for coliforms uses Violet Red Bile Agar, (VRBA). If typical coliform colonies appear, they are tested further to confirm their identification as coliforms. Coliform colonies lower the pH of the medium, subsequently causing their colonies to look red (Neutral Red Dye) and to precipitate the bile salts.
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