acumedia* Wilkins-Chalgren Broth

• Intended Use: Wilkins-Chalgren Broth is used for the cultivation of anaerobic microorganisms.
• Product Summary and Explanation: Wilkins-Chalgren Broth is derived from Wilkins-Chalgren Agar. This broth can be used for
determining susceptibility of anaerobes to antibiotics. In addition, Wilkins-Chalgren Broth provides good growth of anaerobes.
Wilkins and Chalgren formulated Wilkins-Chalgren Agar for use in determining the minimal inhibitory concentration (MICs) of
antibiotics for anaerobic bacteria by the agar dilution procedure. This medium was selected because it does not require the
addition of blood to support satisfactory growth of most anaerobes. Wilkins-Chalgren Broth is the same formula as Wilkins-
Chalgren Agar with agar omitted.

Anaerobic bacteria cause a variety of human infections including endocarditis, meningitis, wound infections following bowel surgery or trauma, and bacteremia. The survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature.
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