acumedia* YM Broth

• Intended Use: YM Broth is used for the cultivation of fungi.
• Product Summary and Explanation: YM Broth is prepared according to the formulation published by Wickerham. Wickerham
suggested that YM Broth, acidified to pH 3.0 - 4.0, be used as an enrichment medium for yeasts from populations also containing
bacteria and molds. To favor isolation of fermentative species, add a layer of sterile paraffin oil 1 cm deep on the surface of the
inoculated broth. Cultures should be incubated until growth appears, and then streaked onto YM Agar to obtain isolated yeast
colonies. To isolate fermentative and oxidative strains, place acidified inoculated YM Broth on a rotary shaker for 1 to 2 days.
This favors yeast recovery while preventing sporulation of molds.

Media selectivity may be enhanced through acidification or addition of selective agents. YM Broth may be acidified prior to sterilization.
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