BIOAFFINITY MATRICIES

Goat Anti-Mouse IgG (Fc)-Red Separopore 4B is used for immunoprecipitation and Co-immunoprecipitation studies. The red color makes it easy to track pellets containing bound material. Affinity isolated antibody is coupled to cyanogen bromide-activated Red...

Goat (anti-Mouse) IgG Separopore 4B is used for immunoprecipitation and Co-immunoprecipitation studies. It is suitable for antibody purification or to remove unwanted cross-reactivity. Affinity isolated antibody is coupled to cyanogen bromide-activated...

Goat Anti-Rabbit IgG (H+L) -Red Separopore 4B binds rabbit IgG and associated antigen-containing proteins to the red colored beads for (co)-immunoprecipitation experiments. Affinity isolated antibody goat anti-rabbit IgG coupled to cyanogen...

Binds rabbit IgG to the beads. Used for immunoprecipitation and Co-immunoprecipitation studies (to separate the IgGs antigen-containing protein from solution). Affinity isolated goat anti-rabbit IgG coupled to cyanogen bromide-activated Separopore 4B...

Reactive Green 19-Separopore 4B-CL is useful for the isolation of nucleotide enzymes, complement factors, human serum albumin. Immobilized green dye binds from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive green...

GTP-Separopore is used for purification of GTP-dependent enzymes and proteins that bind nucleotides and related molecules. The GTP-Separopore 4B-CL resin comprises GTP attached to agarose beads via its -phosphate. A long hydrophilic spacer (14-atom) is...

Guar Gum Separopore (Agarose) 4B-CL: Guar gum is a Galactomannan polymer derived from the seeds of the legume plant Cyamopsis tetragonolobus.Guar gum based affinity chromatography is used for the purification of galactose binding proteins.Storage:...

Heparin Separopore 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid...

Metal ion-chelating (IDA) Separopore 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity...

IgG (mouse)-Separopore 4B is an affinity chromatography gel matrix consisting of mouse IgG bound to Separopore 4B. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. IgG (mouse)-Separopore 4B is suitable for antibody...

Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore 4B). This matrix is coupled through a stable, leak resistant linkage over a wide...

A complete kit to bind Protein A fusion proteins expressed in E. coli or yeast. Kit consists of affinity chromatography gel matrix, IgG-Separopore 4B, including all the components/buffers that allow for quick, convenient purification of anti-IgG...

Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens by removing antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase...

L-Aspartic Acid-Separopore 4B-CL is used for the purification of retinol, or other L-Aspartic Acid binding proteins. Technical specifications:• Ligand: L-Aspartic acid• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)•...

For the purification of membrane dicarboxylate binding proteins and other proteins with affinity to glutamate. Specifications:• Ligand: L-Glutamic acid• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)• Particle size range:...

Histidine-Separopore 4B-CL can be used in the purification of supercoiled (sc) plasmid DNA (pDNA) from Escherichia coli lysates. This can also be used for the separation of IgG from human plasma and selective purification of bioactive oligouronides such...

L-Lysine-Separopore 4B-CL is used for purification of molecules with biospecific or charge dependent affinity for L-lysine. It is also used for the purification of lysine-binding proteins from serum and to purify plasminogen, plasminogen activator,...

L-Phenylalanine-Separopore 4B-CL is used for the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase. Specifications:• Ligand: L-Phenylalanine• Particle size range: 52 - 165 µm• Molecular weight range:...

-Lactose-Separopore 4B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. -Lactose-Separopore 4B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore 4B-CL may be...

Maltose is coupled with Separopore 4B. Maltose Separopore 4B provides a convenient affinity purification of recombinant proteins tagged with maltose-binding protein (MBP). Maltose Separopore 4B has high binding capacity for MBP-tagged proteins...

N-Acetyl-glucosamine-Separopore 4B-CL is used to purify wheat germ (Triticum vulgaris) lectin, other lectins, and certain other carbohydrate-binding proteins. The purification results in specificity and prolonged biological activity of the protein...

NAD covalently bound to Separopore has a high affinity for NAD-dependent enzymes and dehydrogenases. NAD-Separopore 4B-CL has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins. Technical Specifications:•...

NADP covalently bound to Separopore has a high affinity for NADP-dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP-Separopore 4B-CL twelve times higher than NAD-Separopore 4B-CL. NADP-Separopore 4B-CL has also been used to purify...

Rapid immobilization of antibodies and other proteins to the matrix. Immobilization of ligands through primary amines to purify recombinant proteins. Immobilization of Protein A or Protein G for monoclonal antibody purification. Pre-activated medium for...

Ni2+-chelated Separopore 4B-CL is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to nickel metal ions. Ni2+-chelated Separopore 4B-CL is ideal for purification of...

Applications: Octyl-Separopore 4B-CL is a hydrophobic chromatorgraphy matrix used to separate proteins on the basis of relative hydrophobicity. This is particularly useful for the purification from high ionic strength biological extracts since binding is...

Papain-Separopore 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce...

Phenyl-Separopore 4B-CL is used for hydrophobic interaction chromatography to purify membrane proteins and other proteins with hydrophobic regions. This matrix separates proteins based on the basis of the different hydrophobicities of the proteins...

Poly-L-Lysine-Separopore 4B-CL is used for the purification of negatively charged proteins and biomolecules. Technical Specifications:• Ligand: Poly-L-Lysine• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)• Particle size...

Protein A Blue-Separopore 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads...

Protein-A Red Separopore 4B-MB functions the same as conventional affinity beads, but have enhanced visibility due to red colored Separopore macrobeads. The red colored beads help provide visual monitoring to avoid the accidental loss of beads during...

Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue...

Protein G binds specifically to Fc regions of many mammalian immunoglobulins. Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue...

Reactive Blue 4-Separopore is a dye affinity matrix used to purify proteins based on selective binding interactions between certain protein sites and the dye molecule. Different immobilized dyes bind from 5 to 60% of the proteins in various crude...

The immobilized red dye will bind to a wide variety of NADP+- and NAD+-dependent enzymes, including many kinases, dehydrogenases and transferases. Carboxypeptidase G, interferon, plasminogen and plasminogen activator are examples of other proteins that...

Separopore 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore 2B is a proven base matrix for coupling affinity ligands...

Separopore 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore 2B-CL results in low non-specific interactions and high protein recoveries. Separopore 2B-CL provides an...

Separopore 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore 4B is a proven base matrix for coupling affinity ligands...

Separopore 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore 4B-CL is a proven base matrix for coupling affinity ligands...

Separopore 4B (macrobeads) is a larger sized agarose bead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Technical specifications:• Matrix: Separopore 4B (agarose...